Template:Heidelberg/project/standardization/validation

Software Validation

On top of that we needed a high-throughput validation system for the verification of our calculated aptamers. For this we also used the HRP-mimicking DNAzyme. The aptamers that were predicted by MAWS were fused with the HRP DNAzyme using JAWS to generate a bi-stable system (Fig.4). Thus the activity of the HRP DNAzyme became dependent on the presence of the ligand that can bind to the aptamer. This way we validated our aptamer candidates.