| Lactobacillus plantarum, part of natural microbiota of idli fermentation, was chosen to apply metabolic engineering for production of Vitamin B2.
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Vitamin B2 pathway from Bacillus subtilis was codon optimized for Lactobacillus plantarum and two metabolic bottlenecks were overcame by synthetic promoters to have increased Vitamin B2 production.
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- Successfully cloned whole Vitamin B2 enzymatic pathway in coling strain of E.coli.
- Successfully cloned RibD, RibT25, RibT48, synthetic promoters P48 into Biobrick backbones and therefore generated four new BioBricks.
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