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Background

Aims

Results

Lactobacillus plantarum, part of natural microbiota of idli fermentation, was chosen to apply metabolic engineering for production of Vitamin B2. Vitamin B2 pathway from Bacillus subtilis was codon optimized for Lactobacillus plantarum and two metabolic bottlenecks were overcame by synthetic promoters to have increased Vitamin B2 production.
  • Successfully made an E. coli strain overproducing vitamin B2 .
  • BioBrick submitted to the BioBrick Registry: BBa_K1678001, BBa_K1678002, BBa_K1678003, BBa_K1678004