Team:WHU-China/Results

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Results

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We intend to build a criticality detector and explore the potential application of it. The criticality de-tector, consisting of a light-sensing part and a negative feedback circuit, is designed to generate pulse output when the input exceeds the threshold. The binary system uses serine recombinases to record the number of pulse from the detector. The dosage control system is built to produce spe-cially designed antimicrobial peptide in a pulse manner for treating dental caries. In the result page: 1.Photos of gel electrophoresis (confirming the successful construction of all gene circuit) click here 2. Experiments proving the efficiency of the antimicrobial peptide click here 3. CI binding site insertion with whole plasmid PCR click here

Criticality Detector_Light-Sensing Part

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part: Promoter + Cph8 Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 2612 bp Construction succeeded!

part: RBS + ho1 Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 1083 bp Construction succeeded!

Results

part: RBS + pcyA Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 1107 bp Construction succeeded!

part: RBS + ho1 + RBS + pcyA + terminator Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 2021 bp Construction succeeded!

Results

part: RBS + ho1 + RBS + pcyA Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 1884 bp Construction succeeded!

part: Promoter + RBS + Cph8 + RBS + ho1 + RBS + pcyA + terminator Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 4327 bp Construction succeeded!

Results

part: Pompc+Cl binding site+RBS+GFP+terminator Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 1346 bp Construction succeeded!

part: Pompc+Riboregulator+C1+B0010+Pompc+taRNA+B0010 Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 1550 bp Construction succeeded!

Results

part: Pompc+taRNA+B0010 Colony pcr using primer VF2 and VR Theoretical length (from VF2 to VR) : 598 bp Construction succeeded!

Colony pcr part: APP2 (48bp) part: Bac8c (30) part: CAP (28bp) part: CAP-app2(82bp)

Results

part: CAP-Bac8c (64bp) Colony pcr