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HRP-mimicking DNAzyme

Figure 2: HRP-mimicking DNAzyme

HRP-mimicking DNAzyme folds into a G-quadruplex and binds hemin into it. Upon binding of the hemin to the G-quadruplex the DNAzyme catalyzes the reduction of H₂O₂ to H₂O and a reactive oxygen species and thus result to the activation of a classical HRP substrate like luminol.

The HRP-mimicking DNAzymetravascio1998 is our favorite part and as such we used it as modular part in different setups. It forms a G-quadruplex structure in which hemin can bind (Fig.2). This enables it to catalyze the fission of hydrogen peroxide to water and a reactive oxygen species (ROS). Thus it can be used to catalyze chemiluminescence and a series of colorimetric reactions, known from the horseraddish peroxidase from Amoracia rusticana.