Team:Chalmers-Gothenburg/Protocols

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Protocols

PCR: https://2015.igem.org/File:ChalmersgothenburgPCRProtocol.pdf

PCR Purification

Gel extraction

Gibson Assembly Method

Sticky-end DNA Insert Ligation into Vector DNA

FastDigest Restriction

Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation

Yeast Genomic DNA Extraction LiOAc-SDS

Yeast Transformation by Electroporation

Test of Detection System in Saccharomyces cerevisiae