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Protocols

PCR: https://2015.igem.org/File:ChalmersGothenburgPCRprotocol.pdf

PCR Purification:

Gel extraction:

Gibson Assembly Method:

Sticky-end DNA Insert Ligation into Vector DNA:

FastDigest Restriction:

Fast Simultaneous Plasmid Vector Linearization and Dephosphorylation:

Yeast Genomic DNA Extraction LiOAc-SDS:

Yeast Transformation by Electroporation:

Test of Detection System in Saccharomyces cerevisiae: