Team:Tuebingen/Notebook

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Dronpa-Caged Cre

Ligation of gene blocks (Cre, Cre_sn, Dronpa, HCV, HCV+linker, nluc), linker L6, L9, L12, L15, nDegron and NLS with pSB1C3 and transformation in DH10beta:

pSB-Cre_sn and pSB-Luciferase did not yield correct colonies (transformation repeated), the other constructs resulted in correct colonies

Cre Reporter Cassette

Ligation of gene blocks (tADH, loxP WT, loxP66, loxP71) with pSB1C3 and transformation in DH10beta

pRS Vectors

Mutagenesis primer design pRS313, pRS315, pRS316

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy

Cre-Dronpa

Cre Reporter Cassette

pRS Vectors

Sensors/Promotors

Interlabstudy