Competent E. coli were transformed with 10 µL ligation product and plated on LB plate with chloramphenicol (pSB1C3). Transformation successful.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt GFP Transformation zp72
Psalt GFP Transformation zp72
Transformation was done to introduce the ligated plasmid DNA into E. coli.
To create a salt inducible promotor.
Transformation successful.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>
00:00, 26 Augsut 2015 - 00:00, 26 August 2015