Team:Groningen/Notebook/Psalt GFP Colony PCR zp78
Blue Bio Energy
Psalt GFP Colony PCR (zp78)
A colony PCR was performed to check the insert of a transformed E. coli.
To create a salt inducible promotor.
Gel results show that GFP is integrated into the BBa_K823023 backbone.
Colony PCR
00:00, 29 August 2015 - 00:00, 29 August 2015
White colonies from the plate with transformed E. coli were checked for the right insert with the primers seen in table 1. After PCR, sample was loaded on 1% agarose gel with SERVA DNA Stain G 1:50000. Gel results show the correct insert.
Primer
Sequence
VR
attaccgcctttgagtgagc
VF2
tgccacctgacgtctaagaa
Primer sequences used in colony PCR.