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Timeline

Week 26 - Preparing for take-off

• Inventory of the lab supplies
• Pouring LB Agar plates
• Amplification of the pET-Duet-1 vector
• Assessment of the safety requirements
• Preparing stocks for antibiotics, glycerol, LB & MilliQ

Week 27 - The Clone Wars

• Linearizing pET-Duet-1 for Gibson Assembly
• Our first successful Gibson Assembly!
• Amplification of the pET-Duet-1 vector
• Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning