Attributions
Software + Policies & Practices
• PyMOL360 Educational Tool
Patrick Holec
• Synthetic Biology Web Scraper
Patrick Holec
• Mathematical Modeling
Patrick Holec
Wet Lab
Sarah Lucas, Nicholas Nesbitt, Tanner Cook
Wiki
Patrick Holec, Nicholas Nesbitt, Abhiney Jain, Basem Al-Shayeb
Labwork and General Project Support
Basem Al-Shayeb, Sarah Perdue, Casim Sarkar
Special thanks and acknowledgements to Dr. Mark Held (Intrexon) and Dr. Erin Marasco Cargill for arranging meetings with us to speak about our Policies and Practices and Project.
Figure 1. PCR colony screen of PCSK1-pSB1C3 transformants. Note the banding in each lane at roughly 750 bp, consistent with the second fragment of PCSK1.
Figure 2. PCR colony screen of Ins-pSB1C3 transformants. Banding can be seen at roughly 333 bp, consistent with the full insulin ORF.
Sequencing data received for both PCSK1 and insulin was inconclusive.
Parts List
BBa_K1187001 Human insulin, codon optimized for expression in P. pastoris
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