Template:Team:TU Eindhoven/Timeline HTML

Timeline

Week 26

- Preparing for take-off

• Inventory of the lab supplies
• Pouring LB Agar plates
• Amplification of the pET-Duet-1 vector
• Assessment of the safety requirements
• Preparing stocks for antibiotics, glycerol, LB & MilliQ

Week 27

- The Clone Wars

• Linearizing pET-Duet-1 for Gibson Assembly
• Our first successful Gibson Assembly!
• Amplification of the pET-Duet-1 vector
• Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning