Template:Heidelberg/project/standardization/onthesouthern
On the Southern Blot
Not only can the HRP be used in the ApatBody but the part can be adapted in other systems as well. We could show that the HRP-mimicking DNAzyme can be blotted in a Southern blot and its activity is recovered on the membrane. Thus the HRP-mimicking DNAzyme can be used as an easy and fast readout on a Southern blot. It can be connected to any RNA or DNA via splinted ligation
![](https://static.igem.org/mediawiki/2015/d/d7/Fig4_SplintHRP.png)
Figure 4. DNA or RNA labeled with HRP-mimicking DNAzyme
RNA and DNA could be label with HRP-mimicking DNAzyme via splinted ligation
![](https://static.igem.org/mediawiki/2015/8/8c/Fig5_Validation.png)
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