Template:Heidelberg/project/standardization/Detection
HRP-based Detection
We developed this system further to minimize the background activity by combining the HRP DNAzyme with an F8 ribozyme.
Outlook
Further possibilities
Similarly to the HRP DNAzyme other DNAzymes like the RNA-cleaving DNAzymes 7-18 and 10- 23 DNAzyme can be modified to become ligand dependent.
![](https://static.igem.org/mediawiki/2015/3/3b/Fig6_F8HRPDetec.png)
The activity of the HRP-mimicking DNAzyme is controlled via a strong stem. A part of the stem is cleaved of by a switchable F8 DNAzyme in presence of a specific ligand.