Team:UCLA/Notebook/Honeybee Silk/10 May 2015

Transformation with pET24a

1. Thaw 50uL of chemically competent cells for 10 minutes on ice. 2. Add 1uL of pET expression vector to cells, incubate for 5 minutes. 3. Rescue in 200uL of SOC, incubate at 37C for 1 hour. 4. Plate 100uL on kanamycin plates, spread with beads. 5. Incubate at 37C.