Team:UCLA/Notebook/Honeybee Silk/22 May 2015/

Miniprep and Making of Glycerol Stocks

• Each colony that was grown up in the culture tubes was miniprepped and made into a glycerol stock.

Glycerol Stocks

• 500uL of each sample was combined with 500uL of 30% glycerol stock and stored in the -80C freezer.

Miniprep

• Each sample was miniprepped using the Zymo Miniprep kit and according to the kit's protocol.

OD:

1. 200.95ng/uL
2. 379.01ng/uL
3. 374.83ng/uL
4. 521.92ng/uL