Team:Pitt/Notebook
Notebook
Note: all experiments were done with all members present, unless otherwise noted
June 15, 2015
| Protocol | Notes/results/purpose |
|---|---|
| Transformation of plasmids from Pardee paper | Into DH5a, all transformations successful |
| Grow O/N of DH5a | Competent cell prep (Konstantin Borisov) |
June 16, 2015
| Protocol | Notes/results/purpose |
|---|---|
| O/N of transformations | Miniprep |
| DH5a competent cell prep | 25 tubes of 200ul each, enough to last all summer! (Konstantin Borisov, help from Tatyana Yatsenko) |
June 17, 2015
| Protocol | Notes/results/purpose |
|---|---|
| Transformation of plasmids from iGEM registry | Into new DH5a, all transformations successful |
June 18, 2015
| Protocol | Notes/results/purpose |
|---|---|
| Test PT7 lysate with Pardee paper plasmids | No convenient method of visualization, so we started looking for a plate reader |
June 22, 2015
| Protocol | Notes/results/purpose |
|---|---|
| PCR pSB1C3 with backbone fwd and rev primers | For Gibson assembly, learning process |
| Gel of PCR products | Visualization and purification of pSB1C3 backbone |
| Gel purification | Obtained enough backbone for the rest of the summer! |
June 23, 2015
| Protocol | Notes/results/purpose |
|---|---|
| Gibson assembly of protease parts from IDT, transformation into DH5a | All transformations multiple colonies |
| Transformation of CMU ERT7 into DH5a | Success, faint yellow from YFP seen under blue light |
June 24, 2015
| Protocol | Notes/results/purpose |
|---|---|
| Miniprep and diagnostic gel of Gibson assembly | All 3 had appropriate bands |
| Crude lysate | Original S30 extract protocol, much too dilute due to incorrect weighing |