Team:Penn/Receiver

University of Pennsylvania iGEM

PENN iGEM 2015



RECEIVER

The light producing component of the communication system is initiated by a constitutive promoter (BBa_J23100) and followed by an RBS and the lux box. Expression of the genes in the lux box is responsible for producing luminescence. The box contains the following genes in order: LuxC, D, A, B, E and G. Lux A and B are responsible for encoding two subunits of bacterial luciferase. The genes LuxC, D, and E code for the substrate for the light emitting reaction, tetradecanal. The luxG gene has no known function, however, inclusion of the gene is known to increase light output. The circuit is completed with a stop codon and a terminator.

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