Team:Chalmers-Gothenburg/BioBricks
Contents
- 1 BioBricks
- 1.1 1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2
- 1.2 2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2
- 1.3 3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1
- 1.4 4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA
- 1.5 5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB
BioBricks
1. [http://parts.igem.org/Part:BBa_K1603000 BBa_K1603000]: STE2MAM2
Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from STE2 (Saccharomyces cerevisiae) and Pheromone P-factor receptor MAM2 (Schizosaccharomyces pombe) without its signaling peptide. Allows in vivo detection of the Pheromone P-factor from S.pombe through the Pheromone pathway in </i>S.cerevisiae</i>.
2. [http://parts.igem.org/Part:BBa_K1603001 BBa_K1603001]: pTEF1-pSUC2
The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low ATP levels.
3. [http://parts.igem.org/Part:BBa_K1603002 BBa_K1603002]: pTPI1
Promoter to TPI1.
4. [http://parts.igem.org/Part:BBa_K1603003 BBa_K1603003]: RecA
Recombinase A from Deinococcus radiodurans. Used in DNA-repair mechanisms. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.
5. [http://parts.igem.org/Part:BBa_K1603004 BBa_K1603004]: SSB
Single strand binding protein from Deinococcus radiodurans. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.