Team:William and Mary/Part Collection
Part Collections In deciding which parts to submit to the iGEM Registry we focused on three main aspects. In keeping with our theme of promoter effects on transcription, we created a suite of guide RNAs for the targeted dCas9 repression of 11 of the most frequently used promoters in the Registry. For cells which express a dCas9 protein (link to the fdCas9 part page), the transformation of any of our guide RNA parts will repress the expression of any protein under the associated promoter. We also included a scrambled guide RNA to serve as a negative control for troubleshooting purposes.
(BELOW) An example of repression: fluorescence values for RFP under the R0010 promoter, in cells with and without the R0010 guide RNA”) Integrator Suites Detail1 Detail2 Detail3 Detail4 text text Antibiotic Operons Detail1 Detail2 Detail3 Detail4 text text dCas9s Detail1 Detail2 Detail3 Detail4 text text gRNAs Detail1 Detail2 Detail3 Detail4 text text XFPs Under Various Promoters Detail1 Detail2 Detail3 Detail4 text text G^2 Detail1 Detail2 Detail3 Detail4 text text
