Team:Stanford-Brown/Parts

Our Biobricks So many bricks

See our bricks below!

BioHYDRA BioBricks

Biobrick: BBa_K1692028

cotz-aeBlue-CipA the first step involved cloning a Bacillus construct in Escherichia coli of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state.

Polystyrene Synthesis BioBricks

Biobrick: BBa_K1692007

UbiX with T7 promoter and FLAG tag UbiX is a flavin prenyltransferase that normally plays a role in ubiquinone biosynthesis in E. coli. UbiX transfers a prenyl group from dimethylallyl monophosphate (DMAP) to flavin mononucleotide (FMN), thereby creating a cofactor that happens to be essential to the functionality of FDC. Our genetic construct includes the FDC gene, a T7 inducible promoter, a ribosome binding site, and a FLAG-tag peptide sequence for easy and efficient protein purification. We have sequenced our construct and verified that all these components are indeed present.

Biobrick: BBa_K1692008

Styrene Synthesis Operon Combo Plasmid containing a fusion protein of all three enzymes required to synthesise styrene from the starting organic reagent L-Phenylalanine.

Biobrick: BBa_K1692000

Ferulic acid decarboxylase Ferulic Acid Decarboxylase is used to synthesize styrene from trans-cinnamic acid

P(3HB) Synthesis BioBricks

Biobrick: BBa_K1692020

S. aureus type II PanK (CoaA) Type II pantothenate kinase from Staphylococcus aureus, not sensitive to feedback inhibition from CoA.

Biobrick: BBa_K1692034

Pank + hybrid phaCAB + lysis Construct that was cloned to have P(3HB) producing E. coli cells lyse when induced with Arabinose. This would greatly facilitate P(3HB) extraction. We are currently still testing and characterizing this part.

Biobrick: BBa_K1692023

Ptet + LuxlConstruct that was cloned to have P(3HB) producing E. coli cells lyse after reaching a certain population density using a quorum sensing promoter. This would greatly facilitate P(3HB) extraction. We are currently still testing and characterizing this part.

Biobrick: BBa_K1692024

AutolysisConstruct that was cloned to have P(3HB) producing E. coli cells lyse after reaching a certain population density using a quorum sensing promoter. The difference between this brick BBa_K1692023 is that the basic parts composing this gene are in a different order, which is part of our testing to optimize this system. This would greatly facilitate P(3HB) extraction. We are currently still testing and characterizing this part.

Cellulose Sheets BioBricks

Biobrick: BBa_K1692027

CipACellulose binding domain adapted from Imperial 2014 iGEM team. Last part of our composite part BBa_BBa_K1692028. We removed the illegal EcoR1 site that the Imperial 2014 iGEM team had in their construc.

CRATER Briobricks

Biobrick : BBa_K1692033

RFP gDNA This gene provides the intermediate for synthesizing gRNA targeting RFP (BBa_J04450). When used in conjuction with the CRISPR/Cas system, this gRNA will bind to and digest the RFP plasmid.

Biobrick: BBa_K1692030

amilGFP yellow chromoprotein, RBS and promoterThis plasmid contains the amilGFP yellow chromoprotein gene from part BBa_K1033931 with the RBS and promoter from part BBa_K608002.

Biobrick: BBa_K1692031

meffBlue blue chromoprotein with RBS and promoterThis plasmid contains the meffBlue blue chromoprotein gene found in the BBa_K1033902 part, with the RBS and promoter from part BBa_K608002.

Biobrick: BBa_K1692032

amilCP blue chromoprotein with RBS and promoterThis plasmid contains the amilCP blue chromoprotein gene found in part BBa_K592009 and the RBS and promoter from part BBa_K608002.

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