Team:William and Mary/Notebook
Our Notebook On this page you can view the work we did each month, from June through September. You can also find our protocols. Important Protocols Easy access to our most complex protocols. Detail1 Detail2 Detail3 Detail4 Week 1 (7/1-7/7)
Resuspended all parts from the kit
Designed Gibson assemblies to put various fluorescent proteins under the control of the same promoter, to get noise measurements
Began performing Gibson PCRs
Week 2 (7/8-7/14)
Troubleshot Gibson PCRs
Attempted first Gibson assembly
Designed Gibson assemblies to put together different fluorescent proteins, each under the control of the same promoter
Attempted to Gibson assemble YFP + CFP under control of R0010
Week 3 (7/15-7/21)
Re-attempted Gibson assembly to make a double fluorescent construct with proteins under R0010
Began assembling parts for the Interlab Study
Received gBlocks to assemble dCas9 and some preliminary gRNAs
Began assembling dCas9 and gRNAs
Began learning how to use confocal microscopy
Began assembling different promoters with our fluorescent constructs (to eventually collect noise data for each promoter)
Designed and started the assembly process to make KanR (necessary to integrate parts onto the E. coli genome)
Week 4 (7/22-7/28)
Many of our past assemblies (dCas9, double fluorescent constructs, etc) failed. We continued attempts at these assemblies.
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