Team:IISER Pune/Measurement


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Characterized existing BioBricks

The biobricks BBa_K274110,BBa_K274120,BBa_K274210,BBa_K274220 were characterized as the part of Detection module

BioBrick number Description
BBa_K274110 lacI+pL promoter + Lycopene
BBa_K274120 pBAD/araC promoter + Lycopene
BBa_K274210 lacI+pL promoter + beta-carotene
BBa_K274220 lacI+pL promoter + beta-carotene

Characterization by Microscopy

Microscopy was done using brightfeild microscope with colored camera. Cells were induced at the time of sub-culturing Cells were fixed using Fixation protocol.

Control cells
In NEB Top10 cells
In E.coli MG1655 cells