Team:uOttawa/Description

Project Policy Attributions iGEMuOttawa Parts Collaborations Team

Improvement

Our biobrick, pPGK1Gx, is an improvement to pTRE(4)Gx and pTRE(2)Gx from uOttawa's 2014 project. While the previous parts were GEV-repressible but required rtTA to express, pPGK1Gx has strong, constitutive expression. This part does not require the cell to have rtTA to get a signal, and does not require the user to maintain a steady concentration of anhydrotetracycline or doxycycline to modulate the rtTA activity. This way, rtTA is free to be used as a transcription factor elsewhere in a genetic network.

Not only does this behaviour fit with achieving tri-stability, but it is also ideal for implementing new logic gates with genes. This promoter, being repressible, can be used as a single-input NOT gate with a twist. Its input may be able to induce a positive signal somewhere else.