Team:BIT/Composite Part



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Composite Parts

We really like this composite part, whose part name is "BBa_K1651000". For it really make everyone begin to think carefully that what synthetic can do for our life as well as how can it change our life.
This year, we aim at detecting macromolecule by synthetic ways. Once we decided to carry out our project, there are so many problems and considerations that we must solve or think about. Nowadays, we already have many methods to prevent us from the most serious disease. The most advanced techniques will make confirmation in only several days or even several hours while detecting Hepatitis B, AIDS and many other infectious disease.
However, there are still wide areas, especially in the developing country, which need some new kind of detecting methods for the device now widely used is not portable enough. What's more, it is also not available with a low cost.

So all the considerations comes to one termination that is whether there is a method, which has a low cost as well as portability. We are convinced that by optimizing the natural circuit existing in P. Aeruginosa. Like the real circuit, we use the LasIR and RhlIR system to construct a "sensor+amplifier" in the E. Coli.
This part named BBa_K1651000 is exactly the "amplifier" of the system, which can be significantly activated by levels of small molecules (PAI1) can be satisfied.
The following is our data coming from Laboratory for Bioseparation and Bioanalysis.

< img src="https://static.igem.org/mediawiki/2015/0/00/Amplifier_OD.png" alt="">
< img src="https://static.igem.org/mediawiki/2015/9/98/Amplifier_OD2.png" alt="">
< img src="https://static.igem.org/mediawiki/2015/1/16/Amplifier_OD1.png" alt="">
< img src="https://static.igem.org/mediawiki/2015/3/3c/Amplifier_OD3.png" alt="">