University of Virginia iGEM 2015
IPTG Promoter+RBS+EIIA+TT
BBa_K1600001
BioBrick BBa_1600001serves to increase the uptake of glucose by the cell. This extra glucose will then be polymerized by one of our following BioBrick devices (BBa_K1600001) . This composite BioBrick is composed of BBa_K215000 (IPTG-inducible promoter), BBa_R0034 (RBS) and BBa_K1508003 (EIIA plus promoter).
Promoter+RBS+GlgC+RBS+SacB+TT
BBa_K1600002
BioBrick BBa_1600004 is our composite polymerization circuit combining the functions of BBa_1600002 and BBa_1600003. This BioBrick polymerizes glucose into glycogen, polymerizes levan from fructose, and possesses the kill switch responsible for lysing the cell. This composite BioBrick is composed of BBa_J23110 (promoter), BBa_K118018(RBS and Glgc), BBa_K322921 (RBS and SacB) and BBa_B0015 (double terminator).
EIIBC
BBa_K1600000
BioBrick BBa_K1600000 also serves to increase the uptake of glucose by the cell, helping EIIA. EIIBC is the actual channel so this BioBrick would upregulate the uptake of glucose. The only part on this BioBrick is EIIBC which we extracted from the genome via PCR.