Team:York/Protocols

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Protocols

Lysogeny Broth

Materials

• 10g of tryptone
• 5g of yeast extract
• 10g of NaCl
• 1L of Deionized Water
• 1M NaCl
• 1M KOH

Procedure

1. Use a container with at least double the volume of the liquid that you are making.
2. Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
3. Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter.
4. Autoclave.
5. Store at room temperature or +4°C.