Template:Team:Groningen/CONTENT/PROJECT/Results

Overview of achievements

Developed several genetic constructs which resulted in distinct biofilm phenotypes.

Created salt inducible promoter to control several biofilm genes

Validated both the salt inducible P_proH promoter (BBa_K1597000) and tasA (BBa_K1597002)

A new shuttle vector (BBa_K1597001) was created for integration in the thrC locus in B. subtilis

Used combinations of these constructs to create a biofilm which is ion selective

Demonstrated ion-selectivity of B. subtilis biofilm with our prototype in the real-world

y-PGA was modeled as a Cation Exchange membrane using Molecular Dynamics

Created future scenarios about our project and the use of GMOs.

A card game was developed to teach about synthetic biology in a fun way.

Phenotypical differences after biobrick introduction in B. subtilis