Team:UCLA/Notebook/Recombinant Expression/6 July 2015

• HOME
• TEAM
• PROJECTS
  • Silk Genetics
  • Functionalization
  • Materials Processing
  • Protein Cages
• PARTS
  • Team Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
• NOTEBOOKS
  • Spider Silk Genetics
  • Honeybee Silk
  • Recombinant Silk Functionalization
  • Materials Processing
  • Protein Cages
  • UCLA Measurement Interlab Study
• MEETING NOTES
  • General Meetings
  • Coordinator Meetings
  • Advisor Meetings
• ATTRIBUTIONS
• COLLABORATIONS
• HUMAN PRACTICES
• SAFETY
• MODELING
• MEASUREMENT STUDY
• SOFTWARE
• ENTREPRENEURSHIP

Cell Lysis, continued

Calculations of Reagent Quantities Needed (accounting for inflation of cell pellet weight due to inclusion of Falcon tube mass)

Mass of Cell Pellet	Lysozyme	PMSF Solution	Deoxycholic Acid
0.52g	0.0082g	0.41mL	0.78g
0.32g	0.0082g	0.41mL	0.48g
0.42g	0.0084g	0.42mL	0.63g
0.52g	0.0084g	0.42mL	0.78g
0.42g	0.0084g	0.42mL	0.63g
0.52g	0.0084g	0.42mL	0.78g
0.02g	0.0084g	0.42mL	0.03g
0.52g	0.0084g	0.42mL	0.78g
0.82g	0.0084g	0.42mL	1.23g
0.32g	0.0084g	0.42mL	0.48g
0.32g	0.0084g	0.42mL	0.48g
0.42g	0.0084g	0.42mL	0.63g
0.22g	0.0084g	0.42mL	0.33g
0.52g	0.0084g	0.42mL	0.78g
0.62g	0.0084g	0.42mL	0.93g
0.42g	0.0084g	0.42mL	0.63g
0.32g	0.0084g	0.42mL	0.48g
0.12g	0.0084g	0.42mL	0.18g
0.62g	0.0084g	0.42mL	0.93g
0.52g	0.0084g	0.42mL	0.78g