Template:Team:TU Eindhoven/Timeline HTML
Timeline
Week 26
- Preparing for take-off
Gibson Assembly
- Inventory of the lab supplies
- Pouring LB Agar plates
- Amplification of the pET-Duet-1 vector
- Assessment of the safety requirements
- Preparing stocks for antibiotics, glycerol, LB & MilliQ
Week 27
- The Clone Wars
- Linearizing pET-Duet-1 for Gibson Assembly
- Our first Gibson Assembly!
- Amplification of the pET-Duet-1 vector
- Nde1 & Kpn1 digestion of the pET-Duet-1 vector (MCS-2) for traditional cloning
Week 28
- Et tu, pET-Duet?
- Linearizing pET-Duet-1 for Gibson Assembly and debugging
- Amplification of the inserts for traditional cloning
- Xbal & Pstl digestion of the pET-Duet-1 vector (MCS-1) for traditional cloning
- Xba1 & Pst1 digestion of the inserts (MCS-1) for traditional cloning
- Nde1 & Kpn1 digestion of the inserts (MCS-2) for traditional cloning
- Ligation