Team:Vanderbilt/Project/Achievements

Vanderbilt iGEM 2015

Sequence

Invented an innovative software tool for minimizing any gene’s susceptibility to mutation
Validated rapid spread of mutants in a genetically modified population by mathematical and empirical techniques
Experimentally validated decreased mutation in optimized sequences with

Multiple mutagen types (UV radiation, oxidation)
Multiple quantification protocols (Alkaline gel, plasmid conformation, PCR inhibition)

Established high expression of optimized sequences
Modeling and computational strategies for further improvements and expansion of software
Investigated potential for nanopore sequencing to become next-generation of ultra high-throughput DNA damage detection

Introduced quantitative metric of expected evolutionary stability and computationally modeled simple circuits
Invented software tool to analyze circuit designs, calculate stability, and suggest modifications to improvements
Developed assays for measuring decreased recombination with homology-minimization software
Constructed optimized circuit to demonstrate improved stability with VERT technique
Validated bidirectional promoter for use with system to select against promoter mutation

Cloned five exogenous DNA repair enzymes
Incorporated KIKO vector for genomic integration and simultaneous knock-out
Designed “Incorruptible Cell” that commits suicide instead of passing on mutations

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