Template:Team:Groningen/CONTENT/PAE/treeall

[ { "date":"05-09-2015", "title":"tasA Restriction", "category":"tb", "number": 1, "isroot": true, "next": ["tb3"] }, { "date":"05-09-2015", "title":"tasA bslA ligation", "category":"tb", "number": 3, "isroot": false, "next": ["tb4"] }, { "date":"05-09-2015", "title":"bslA Restriction", "category":"tb", "number": 2, "isroot": true, "next": ["tb3"] }, { "date":"05-09-2015", "title":"tasA bslA Transformation", "category":"tb", "number": 4, "isroot": false, "next": null }, { "date":"06-09-2015", "title":"tasA Restriction", "category":"tb", "number": 5, "isroot": true, "next": ["tb7"] }, { "date":"06-09-2015", "title":"tasA bslA ligation", "category":"tb", "number": 7, "isroot": false, "next": ["tb8"] }, { "date":"06-09-2015", "title":"bslA Restriction", "category":"tb", "number": 6, "isroot": true, "next": ["tb8"] }, { "date":"06-09-2015", "title":"tasA bslA Transformation", "category":"tb", "number": 8, "isroot": false, "next": ["tb9"] }, { "date":"07-09-2015", "title":"tasA bslA Colony PCR", "category":"tb", "number": 9, "isroot": false, "next": ["tb10"] }, { "date":"08-09-2015", "title":"tasA bslA Miniprep", "category":"tb", "number": 10, "isroot": false, "next": ["tb11"] }, { "date":"08-09-2015", "title":"tasA bslA Transformation", "category":"tb", "number": 11, "isroot": false, "next": [] }, { "date":"22-07-2015", "title":"Liquid Medium", "category":"co", "number": 1, "isroot": true, "next": ["co2","co3"] }, { "date":"22-07-2015", "title":"CFR", "category":"co", "number": 2, "isroot": false, "next": null }, { "date":"24-07-2015", "title":"Centrifugation", "category":"co", "number": 3, "isroot": false, "next": ["co4"] }, { "date":"24-07-2015", "title":"Dissolve in PBS", "category":"co", "number": 4, "isroot": false, "next": ["co5"] }, { "date":"24-07-2015", "title":"CFR", "category":"co", "number": 5, "isroot": false, "next": null }, { "date":"20-07-2015", "title":"CFR", "category":"Liquid Medium", "number": 7, "isroot": true, "next": ["co8"] }, { "date":"21-07-2015", "title":"Plate", "category":"co", "number": 8, "isroot": false, "next": ["co9"] }, { "date":"22-07-2015", "title":"DFR", "category":"co", "number": 9, "isroot": false, "next": null }, { "date":"23-07-2015", "title":"Liquid Medium", "category":"co", "number": 10, "isroot": true, "next": ["co11"] }, { "date":"24-07-2015", "title":"Plate", "category":"co", "number": 11, "isroot": false, "next": ["co12"] }, { "date":"25-07-2015", "title":"DFR", "category":"co", "number": 12, "isroot": false, "next": null }, { "date":"29-07-2015", "title":"Liquid Medium", "category":"co", "number": 15, "isroot": true, "next": ["co16","co17"] }, { "date":"30-07-2015", "title":"Plate", "category":"co", "number": 16, "isroot": false, "next": ["co17"] }, { "date":"30-07-2015", "title":"Liquid Medium", "category":"co", "number": 17, "isroot": false, "next": ["co21"] }, { "date":"01-08-2015", "title":"Liquid Medium", "category":"co", "number": 18, "isroot": true, "next": ["co19"] }, { "date":"03-08-2015", "title":"Plated", "category":"co", "number": 19, "isroot": false, "next": ["co20"] }, { "date":"05-08-2015", "title":"DFR", "category":"co", "number": 20, "isroot": false, "next": null }, { "date":"05-08-2015", "title":"Plates", "category":"co", "number": 21, "isroot": false, "next": null }, { "date":"15-08-2015", "title":"Transformation with RFP", "category":"co", "number": 22, "isroot": true, "next": null }, { "date":"04-08-2015", "title":"Transformation with GFP", "category":"co", "number": 23, "isroot": true, "next": null }, { "date":"22-08-2015", "title":"Transformation with GFP", "category":"co", "number": 24, "isroot": true, "next": null },

       {

"date":"15-08-2015", "title":"TSB 2.0 vs TSBstar", "category":"ab", "number": 1, "isroot": true, "next": null }, { "date":"09-09-2015", "title":"Transform comI", "category":"ab", "number": 2, "isroot": true, "next": ["ab3"] }, { "date":"11-09-2015", "title":"Transform with slrR", "category":"ab", "number": 3, "isroot": false, "next": ["ab4"] }, { "date":"29-08-2015", "title":"Streak Plates", "category":"ab", "number": 4, "isroot": false, "next": ["ab5","ab6","ab7"] }, { "date":"01-09-2015", "title":"New Streak Plates", "category":"ab", "number": 5, "isroot": false, "next": [] }, { "date":"30-08-2015", "title":"New Streak Plates", "category":"ab", "number": 6, "isroot": false, "next": [] }, { "date":"03-09-2015", "title":"JH642 Streak Plate", "category":"ab", "number": 7, "isroot": false, "next": [] }, { "date":"29-08-2015", "title":"Transform Natto", "category":"ab", "number": 8, "isroot": true, "next": [] }, { "date":"14-08-2015", "title":"Transform JH642, PY79", "category":"ab", "number": 9, "isroot": false, "next": ["ab4"] }, { "date":"26-08-2015", "title":"Transform JH642, PY79, comI", "category":"ab", "number": 10, "isroot": false, "next": ["ab4"] }, { "date":"25-08-2015", "title":"Transform JH642, PY79", "category":"ab", "number": 11, "isroot": false, "next": ["ab4"] }, { "date":"14-08-2015", "title":"Transform JH642, PY79", "category":"ab", "number": 12, "isroot": false, "next": ["ab4"] }, { "date":"28-08-2015", "title":"PCR", "category":"ab", "number": 13, "isroot": true, "next": ["ab14"] }, { "date":"29-08-2015", "title":"A2 Recombination", "category":"ab", "number": 14, "isroot": false, "next": ["ab15"] }, { "date":"29-08-2015", "title":"Restriction", "category":"ab", "number": 15, "isroot": false, "next": ["ab16"] }, { "date":"29-08-2015", "title":"Ligation", "category":"ab", "number": 16, "isroot": false, "next": [] },

       {

"date":"28-08-2015", "title":"PCR", "category":"a", "number": 17, "isroot": false, "next": ["a18"] }, { "date":"29-08-2015", "title":"A1 Recombination", "category":"a", "number": 18, "isroot": false, "next": ["a19"] }, { "date":"29-08-2015", "title":"Restriction", "category":"a", "number": 19, "isroot": false, "next": ["a20"] }, { "date":"29-08-2015", "title":"Ligation", "category":"a", "number": 20, "isroot": false, "next": [] }, { "date":"03-09-2015", "title":"PCR", "category":"a", "number": 21, "isroot": false, "next": ["a23"] }, { "date":"05-09-2015", "title":"Digestion", "category":"a", "number": 23, "isroot": false, "next": ["a24"] }, { "date":"05-09-2015", "title":"Ligation", "category":"a", "number": 24, "isroot": false, "next": ["a25"] }, { "date":"06-09-2015", "title":"Transform E. coli", "category":"a", "number": 25, "isroot": false, "next": null }, { "date":"02-09-2015", "title":"PCR", "category":"a", "number": 26, "isroot": false, "next": null }, { "date":"26-07-2015", "title":"Transformation", "category":"a", "number": 27, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"Rigidity OD", "category":"a", "number": 29, "isroot": false, "next": null }, { "date":"08-08-2015", "title":"Transformation", "category":"a", "number": 30, "isroot": false, "next": ["a29","a32","a34","a35","a39","a40","a41"] }, { "date":"17-08-2015", "title":"OCV", "category":"a", "number": 31, "isroot": false, "next": [] }, { "date":"15-08-2015", "title":"Transformation", "category":"a", "number": 32, "isroot": false, "next": ["a31","a33"] }, { "date":"16-08-2015", "title":"OCV", "category":"a", "number": 33, "isroot": false, "next": [] }, { "date":"25-07-2015", "title":"Rigidity cfu", "category":"a", "number": 34, "isroot": false, "next": [] }, { "date":"24-07-2015", "title":"Rigidity OD", "category":"a", "number": 35, "isroot": false, "next": [] }, { "date":"25-07-2015", "title":"bd1807 Purification", "category":"a", "number": 36, "isroot": true, "next": ["a27","a30","a26","a17","a21","a41"] }, { "date":"10-08-2015", "title":"128 Submerged Plates", "category":"a", "number": 37, "isroot": true, "next": [] }, { "date":"20-08-2015", "title":"abrB Submerged Plates", "category":"a", "number": 38, "isroot": true, "next": [] }, { "date":"30-08-2015", "title":"30 vs 37", "category":"a", "number": 39, "isroot": false, "next": null }, { "date":"31-08-2015", "title":"30 vs 37", "category":"a", "number": 40, "isroot": false, "next": [] }, { "date":"08-09-2015", "title":"PCR", "category":"a", "number": 41, "isroot": false, "next": [] }, { "date":"31-08-2015", "title":"PCR", "category":"fr", "number": 1, "isroot": true, "next": ["fr1"] }, { "date":"31-08-2015", "title":"Purification", "category":"fr", "number": 2, "isroot": false, "next": ["fr2"] }, { "date":"31-08-2015", "title":"Ligation", "category":"fr", "number": 3, "isroot": false, "next": ["fr4"] }, { "date":"31-08-2015", "title":"Gel Purification", "category":"fr", "number": 4, "isroot": false, "next": ["fr5"] }, { "date":"31-08-2015", "title":"Ligation", "category":"fr", "number": 5, "isroot": false, "next": ["fr6"] }, { "date":"31-08-2015", "title":"Transformation", "category":"fr", "number": 6, "isroot": false, "next": ["fr7"] }, { "date":"01-09-2015", "title":"Colony PCR", "category":"fr", "number": 7, "isroot": false, "next": ["fr8"] }, { "date":"02-09-2015", "title":"Transformation", "category":"fr", "number": 8, "isroot": false, "next": ["fr9"] }, { "date":"02-09-2015", "title":"Miniprep", "category":"fr", "number": 9, "isroot": false, "next": [] }, { "date":"02-09-2015", "title":"Glycerol Culture", "category":"fr", "number": 10, "isroot": false, "next": ["fr11"] }, { "date":"11-09-2015", "title":"IPTG", "category":"fr", "number": 11, "isroot": false, "next": [] }, { "date":"17-08-2015", "title":"128 Plates", "category":"py", "number": 1, "isroot": true, "next": ["py2"] }, { "date":"17-08-2015", "title":"LB+G Wet", "category":"py", "number": 2, "isroot": false, "next": null }, { "date":"07-08-2015", "title":"CFR test", "category":"py", "number": 3, "isroot": true, "next": null }, { "date":"09-08-2015", "title":"DFR test", "category":"py", "number": 4, "isroot": true, "next": null }, { "date":"15-08-2015", "title":"TSB 2.0 vs TSB*", "category":"py", "number": 5, "isroot": true, "next": null }, { "date":"15-08-2015", "title":"Transform with rpf", "category":"py", "number": 6, "isroot": true, "next": null }, { "date":"11-08-2015", "title":"Partially Submerged Growth", "category":"py", "number": 7, "isroot": true, "next": null

       },

{ "date":"23-08-2015", "title":"Plates", "category":"n", "number": 1, "isroot": true, "next": ["n2","n4"] }, { "date":"09-09-2015", "title":"Kickstarters", "category":"n", "number": 2, "isroot": false, "next": ["n3"] }, { "date":"09-09-2015", "title":"OCV on Coffee Filters", "category":"n", "number": 3, "isroot": false, "next": null },

       {

"date":"25-08-2015", "title":"OCV on w2", "category":"n", "number": 4, "isroot": false, "next": null }, { "date":"10-08-2015", "title":"Gibson Assembly", "category":"pg", "number": 30, "isroot": true, "next": ["pg31"] }, { "date":"10-08-2015", "title":"PCR", "category":"pg", "number": 31, "isroot": false, "next": null }, { "date":"14-07-2015", "title":"PCR", "category":"pg", "number": 1, "isroot": true, "next": ["pg2"] }, { "date":"15-07-2015", "title":"Restriction", "category":"pg", "number": 2, "isroot": false, "next": ["pg4"] }, { "date":"15-07-2015", "title":"Purification", "category":"pg", "number": 4, "isroot": false, "next": ["pg5"] }, { "date":"15-07-2015", "title":"Ligation", "category":"pg", "number": 5, "isroot": false, "next": ["pg6"] }, { "date":"15-07-2015", "title":"Transformation", "category":"pg", "number": 6, "isroot": false, "next": ["pg7","pg8"] }, { "date":"15-07-2015", "title":"Colony PCR", "category":"pg", "number": 7, "isroot": false, "next": null }, { "date":"18-07-2015", "title":"Colony PCR", "category":"pg", "number": 8, "isroot": false, "next": null }, { "date":"28-07-2015", "title":"Overlap PCR", "category":"pg", "number": 21, "isroot": true, "next": ["pg22"] }, { "date":"31-07-2015", "title":"Purification", "category":"pg", "number": 22, "isroot": false, "next": ["pg24"] }, { "date":"31-07-2015", "title":"Digestion", "category":"pg", "number": 24, "isroot": false, "next": ["pg25"] }, { "date":"31-07-2015", "title":"Ligation in pSB1C3", "category":"pg", "number": 25, "isroot": false, "next": ["pg26"] }, { "date":"31-07-2015", "title":"Transformation with psB1C3", "category":"pg", "number": 26, "isroot": false, "next": ["pg27"] }, { "date":"03-08-2015", "title":"Colony PCR", "category":"pg", "number": 27, "isroot": false, "next": null }, { "date":"20-07-2015", "title":"PCR", "category":"pg", "number": 9, "isroot": true, "next": null }, { "date":"21-07-2015", "title":"PCR", "category":"pg", "number": 10, "isroot": true, "next": ["pg11","pg28"] }, { "date":"22-07-2015", "title":"Purification", "category":"pg", "number": 11, "isroot": false, "next": ["pg12","pg29"] }, { "date":"22-07-2015", "title":"Restriction", "category":"pg", "number": 12, "isroot": false, "next": ["pg13"] }, { "date":"22-07-2015", "title":"Purification", "category":"pg", "number": 13, "isroot": false, "next": ["pg14"] }, { "date":"22-07-2015", "title":"Ligation Backbone", "category":"pg", "number": 14, "isroot": false, "next": ["pg15","pg18"] }, { "date":"22-07-2015", "title":"Transformation", "category":"pg", "number": 15, "isroot": false, "next": ["pg16"] }, { "date":"23-07-2015", "title":"Colony PCR", "category":"pg", "number": 16, "isroot": false, "next": null }, { "date":"06-08-2015", "title":"PCR", "category":"pg", "number": 29, "isroot": false, "next": null }, { "date":"25-07-2015", "title":"PCR", "category":"pg", "number": 18, "isroot": false, "next": ["pg17"] }, { "date":"25-07-2015", "title":"Transformation", "category":"pg", "number": 17, "isroot": false, "next": ["pg19","pg20"] }, { "date":"31-07-2015", "title":"Colony PCR", "category":"pg", "number": 19, "isroot": false, "next": null }, { "date":"26-07-2015", "title":"Colony PCR", "category":"pg", "number": 20, "isroot": false, "next": null }, { "date":"04-08-2015", "title":"Transformation", "category":"pg", "number": 28, "isroot": false, "next": null }, { "date":"28-06-2015", "title":"Psalt PCR", "category":"zp", "number": 1, "isroot": true, "next": ["zp2"] }, { "date":"29-06-2015", "title":"Psalt Digestion", "category":"zp", "number": 2, "isroot": false, "next": ["zp3"] }, { "date":"29-06-2015", "title":"Psalt Ligation", "category":"zp", "number": 3, "isroot": false, "next": ["zp4"] }, { "date":"29-06-2015", "title":"Psalt Transformation", "category":"zp", "number": 4, "isroot": false, "next": null }, { "date":"30-06-2015", "title":"Psalt PCR", "category":"zp", "number": 5, "isroot": true, "next":["zp6","zp10","zp14","zp21","zp24"] }, { "date":"09-07-2015", "title":"Psalt Digestion", "category":"zp", "number": 6, "isroot": false, "next": ["zp7"] }, { "date":"09-07-2015", "title":"Psalt PCR purification", "category":"zp", "number": 7, "isroot": false, "next": ["zp8"] }, { "date":"09-07-2015", "title":"Psalt Ligation", "category":"zp", "number": 8, "isroot": false, "next": ["zp9"] }, { "date":"09-07-2015", "title":"Psalt Transformation", "category":"zp", "number": 9, "isroot": false, "next": null }, { "date":"10-07-2015", "title":"Psalt Digestion", "category":"zp", "number": 10, "isroot": false, "next": ["zp11"] }, { "date":"10-07-2015", "title":"Psalt PCR purification", "category":"zp", "number": 11, "isroot": false, "next": ["zp12"] }, { "date":"10-07-2015", "title":"Psalt Ligation", "category":"zp", "number": 12, "isroot": false, "next": ["zp13"] }, { "date":"10-07-2015", "title":"Psalt Transformation", "category":"zp", "number": 13, "isroot": false, "next": null }, { "date":"13-07-2015", "title":"Psalt Digestion", "category":"zp", "number": 14, "isroot": false, "next": ["zp15"] }, { "date":"13-07-2015", "title":"Psalt PCR purification", "category":"zp", "number": 15, "isroot": false, "next": ["zp16"] }, { "date":"13-07-2015", "title":"Psalt Ligation", "category":"zp", "number": 16, "isroot": false, "next": ["zp17"] }, { "date":"13-07-2015", "title":"Psalt Transformation", "category":"zp", "number": 17, "isroot": false, "next": ["zp18","zp19","zp20"] }, { "date":"15-07-2015", "title":"Psalt Colony PCR", "category":"zp", "number": 18, "isroot": false, "next": null }, { "date":"16-07-2015", "title":"Psalt Colony PCR", "category":"zp", "number": 19, "isroot": false, "next": null }, { "date":"17-07-2015", "title":"Psalt Colony PCR", "category":"zp", "number": 20, "isroot": false, "next": null }, { "date":"20-07-2015", "title":"Psalt Digestion", "category":"zp", "number": 21, "isroot": false, "next": ["zp22"] }, { "date":"20-07-2015", "title":"Psalt Ligation", "category":"zp", "number": 22, "isroot": false, "next": ["zp23"] }, { "date":"20-07-2015", "title":"Psalt Transformation", "category":"zp", "number": 23, "isroot": false, "next": null }, { "date":"21-07-2015", "title":"Psalt Digestion", "category":"zp", "number": 24, "isroot": false, "next": ["zp25"] }, { "date":"21-07-2015", "title":"Psalt Purification", "category":"zp", "number": 25, "isroot": false, "next": ["zp26"] }, { "date":"21-07-2015", "title":"Psalt Ligation", "category":"zp", "number": 26, "isroot": false, "next": ["zp27"] }, { "date":"21-07-2015", "title":"Psalt Transformation", "category":"zp", "number": 27, "isroot": false, "next": null }, { "date":"24-07-2015", "title":"Psalt New Primers", "category":"zp", "number": 28, "isroot": true, "next": ["zp29"] }, { "date":"16-08-2015", "title":"Psalt PCR", "category":"zp", "number": 29, "isroot": false, "next": ["zp30"] }, { "date":"17-08-2015", "title":"Psalt Digestion", "category":"zp", "number": 30, "isroot": false, "next": ["zp31"] }, { "date":"17-08-2015", "title":"Psalt Purification", "category":"zp", "number": 31, "isroot": false, "next": ["zp32"] }, { "date":"17-08-2015", "title":"Psalt Ligation", "category":"zp", "number": 32, "isroot": false, "next": ["zp33"] }, { "date":"17-08-2015", "title":"Psalt Transformation", "category":"zp", "number": 33, "isroot": false, "next": ["zp34"] }, { "date":"18-08-2015", "title":"Psalt Colony PCR", "category":"zp", "number": 34, "isroot": false, "next": ["zp35"] }, { "date":"19-08-2015", "title":"Psalt Miniprep", "category":"zp", "number": 35, "isroot": false, "next": ["zp36","zp53","zp62"] }, { "date":"19-08-2015", "title":"Psalt Digestion", "category":"zp", "number": 36, "isroot": false, "next": ["zp37"] }, { "date":"19-08-2015", "title":"Psalt Purification", "category":"zp", "number": 37, "isroot": false, "next": ["zp38","zp46","zp48"] }, { "date":"20-08-2015", "title":"Psalt Ligation", "category":"zp", "number": 38, "isroot": false, "next": ["zp101"] }, { "date":"20-09-2015", "title":"Psalt Transformation", "category":"zp", "number": 101, "isroot": false, "next": null }, { "date":"17-08-2015", "title":"GFP Digestion", "category":"zp", "number": 39, "isroot": true, "next": ["zp40"] }, { "date":"17-08-2015", "title":"GFP Ligation", "category":"zp", "number": 40, "isroot": false, "next": ["zp41"] }, { "date":"17-08-2015", "title":"GFP Transformation", "category":"zp", "number": 41, "isroot": false, "next": ["zp42"] }, { "date":"18-08-2015", "title":"GFP Colony PCR", "category":"zp", "number": 42, "isroot": false, "next": ["zp43"] }, { "date":"19-08-2015", "title":"GFP Miniprep", "category":"zp", "number": 43, "isroot": false, "next": ["zp44","zp50"] }, { "date":"19-08-2015", "title":"GFP Digestion", "category":"zp", "number": 44, "isroot": false, "next": ["zp38"] }, { "date":"24-08-2015", "title":"Psalt Digestion GFP", "category":"zp", "number": 50, "isroot": false, "next": ["zp51"] }, { "date":"24-08-2015", "title":"Psalt GFP Purification", "category":"zp", "number": 51, "isroot": false, "next": ["zp60","zp103"] }, { "date":"20-08-2015", "title":"Psalt Ligation tasA", "category":"zp", "number": 48, "isroot": false, "next": ["zp49"] }, { "date":"20-08-2015", "title":"Psalt tasA Transformation", "category":"zp", "number": 49, "isroot": false, "next": null }, { "date":"20-08-2015", "title":"Psalt Ligation bslA", "category":"zp", "number": 46, "isroot": false, "next": ["zp47"] }, { "date":"20-08-2015", "title":"Psalt bslA Transformation", "category":"zp", "number": 47, "isroot": false, "next": null }, { "date":"24-08-2015", "title":"Psalt Digestion", "category":"zp", "number": 53, "isroot": false, "next": ["zp60","zp54","zp57"] }, { "date":"24-08-2015", "title":"Psalt Ligation bslA", "category":"zp", "number": 57, "isroot": false, "next": ["zp58"] }, { "date":"24-08-2015", "title":"Psalt bslA Transformation", "category":"zp", "number": 58, "isroot": false, "next": ["zp59"] }, { "date":"25-08-2015", "title":"Psalt bslA Miniprep", "category":"zp", "number": 59, "isroot": false, "next": null }, { "date":"24-08-2015", "title":"Psalt Ligation tasA", "category":"zp", "number": 54, "isroot": false, "next": ["zp55"] }, { "date":"24-08-2015", "title":"Psalt tasA Transformation", "category":"zp", "number": 55, "isroot": false, "next": ["zp56"] }, { "date":"25-08-2015", "title":"Psalt tasA Miniprep", "category":"zp", "number": 56, "isroot": false, "next": null }, { "date":"24-08-2015", "title":"Psalt Ligation GFP", "category":"zp", "number": 60, "isroot": false, "next": ["zp61"] }, { "date":"24-08-2015", "title":"Psalt GFP Transformation", "category":"zp", "number": 61, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"Psalt Digestion", "category":"zp", "number": 62, "isroot": false, "next": ["zp71","zp69","zp63"] }, { "date":"26-08-2015", "title":"Psalt Ligation bslA", "category":"zp", "number": 63, "isroot": false, "next": ["zp64"] }, { "date":"26-08-2015", "title":"Psalt bslA Transformation", "category":"zp", "number": 64, "isroot": false, "next": ["zp68"] }, { "date":"28-08-2015", "title":"Psalt bslA Miniprep", "category":"zp", "number": 65, "isroot": false, "next": ["zp83"] }, { "date":"29-08-2015", "title":"Psalt bslA Colony PCR", "category":"zp", "number": 68, "isroot": false, "next": ["65"] }, { "date":"26-08-2015", "title":"Psalt tasA Ligation", "category":"zp", "number": 69, "isroot": false, "next": ["zp70"] }, { "date":"26-08-2015", "title":"Psalt tasA Transformation", "category":"zp", "number": 70, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"Psalt GFP Ligation", "category":"zp", "number": 71, "isroot": false, "next": ["zp72"] }, { "date":"28-08-2015", "title":"Psalt GFP Transformation", "category":"zp", "number": 72, "isroot": false, "next": ["zp73"] }, { "date":"28-08-2015", "title":"Psalt GFP Miniprep", "category":"zp", "number": 73, "isroot": false, "next": ["zp74"] }, { "date":"28-08-2015", "title":"Psalt GFP Digestion", "category":"zp", "number": 74, "isroot": false, "next": ["zp75"] }, { "date":"28-08-2015", "title":"Psalt GFP Purification", "category":"zp", "number": 75, "isroot": false, "next": ["zp76"] }, { "date":"28-08-2015", "title":"Psalt GFP Ligation", "category":"zp", "number": 76, "isroot": false, "next": ["zp77"] }, { "date":"28-08-2015", "title":"Psalt GFP Transformation", "category":"zp", "number": 77, "isroot": false, "next": ["zp78"] }, { "date":"29-08-2015", "title":"Psalt GFP Colony PCR", "category":"zp", "number": 78, "isroot": false, "next": ["zp79"] }, { "date":"30-08-2015", "title":"Psalt GFP Miniprep", "category":"zp", "number": 79, "isroot": false, "next": ["zp80"] }, { "date":"31-08-2015", "title":"Psalt GFP Transformation", "category":"zp", "number": 80, "isroot": false, "next": ["zp81","zp82"] }, { "date":"01-09-2015", "title":"Psalt GFP Validation", "category":"zp", "number": 81, "isroot": false, "next": null }, { "date":"02-09-2015", "title":"Psalt GFP Validation", "category":"zp", "number": 82, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"GFP Digestion", "category":"zp", "number": 103, "isroot": false, "next": ["zp102"] }, { "date":"26-08-2015", "title":"GFP Purification", "category":"zp", "number": 102, "isroot": false, "next": ["zp71"] }, { "date":"04-09-2015", "title":"Psalt Validation", "category":"zp", "number": 83, "isroot": false, "next": [] },{ "date":"28-07-2015", "title":"slrR PCR", "category":"sl", "number": 1, "isroot": true, "next": ["sl2","sl6"] }, { "date":"29-07-2015", "title":"slrR Restriction", "category":"sl", "number": 2, "isroot": false, "next": ["sl3"] }, { "date":"29-07-2015", "title":"slrR PCR Purification", "category":"sl", "number": 3, "isroot": false, "next": ["sl4","sl28"] }, { "date":"29-07-2015", "title":"slrR Ligation in pSB1C3", "category":"sl", "number": 28, "isroot": false, "next": ["sl29"] }, { "date":"30-07-2015", "title":"SlrR Transformation", "category":"sl", "number": 29, "isroot": false, "next": [] }, { "date":"29-07-2015", "title":"SlrR Ligation in K823023", "category":"sl", "number": 4, "isroot": false, "next": ["sl5"] }, { "date":"30-07-2015", "title":"SlrR Transformation", "category":"sl", "number": 5, "isroot": false, "next": null }, { "date":"17-08-2015", "title":"SlrR Restriction", "category":"sl", "number": 6, "isroot": false, "next": ["sl7"] }, { "date":"17-08-2015", "title":"SlrR PCR Purification", "category":"sl", "number": 7, "isroot": false, "next": ["sl8"] }, { "date":"17-08-2015", "title":"SlrR Ligation in K823023", "category":"sl", "number": 8, "isroot": false, "next": ["sl9"] }, { "date":"17-08-2015", "title":"SlrR Transformation", "category":"sl", "number": 9, "isroot": false, "next": ["sl10"] }, { "date":"18-08-2015", "title":"SlrR Colony PCR", "category":"sl", "number": 10, "isroot": false, "next": ["sl11"] }, { "date":"19-08-2015", "title":"SlrR Miniprep", "category":"sl", "number": 11, "isroot": false, "next": ["sl12","sl16","sl20","sl13","sl17","sl90","sl14","sl21","sl18","sl24"] }, { "date":"25-08-2015", "title":"SlrR Transformation 3610 JH642", "category":"sl", "number": 12, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"SlrR Transformation PY79", "category":"sl", "number": 16, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"SlrR Transformation JH642", "category":"sl", "number": 20, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"SlrR Transformation 3610 comI", "category":"sl", "number": 13, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"SlrR Transformation PY79", "category":"sl", "number": 17, "isroot": false, "next": null }, { "date":"26-08-2015", "title":"SlrR Transformation JH642", "category":"sl", "number": 90, "isroot": false, "next": null }, { "date":"27-08-2015", "title":"SlrR Transformation PY79", "category":"sl", "number": 18, "isroot": false, "next": ["sl19"] }, { "date":"27-08-2015", "title":"SlrR Transformation JH642", "category":"sl", "number": 21, "isroot": false, "next": ["sl22","sl23"] }, { "date":"26-08-2015", "title":"SlrR Transformation 3610 comI", "category":"sl", "number": 14, "isroot": false, "next": ["sl15"] }, { "date":"29-08-2015", "title":"SlrR Streak plate 3610 comI", "category":"sl", "number": 15, "isroot": false, "next": [] }, { "date":"29-08-2015", "title":"SlrR Streak plate PY79", "category":"sl", "number": 19, "isroot": false, "next": null }, { "date":"29-08-2015", "title":"SlrR Streak plate JH642", "category":"sl", "number": 22, "isroot": false, "next": null }, { "date":"30-08-2015", "title":"SlrR Streak plate JH642", "category":"sl", "number": 23, "isroot": false, "next": null }, { "date":"29-08-2015", "title":"SlrR Transformation Natto", "category":"sl", "number": 24, "isroot": false, "next": ["sl25","sl26"] }, { "date":"01-09-2015", "title":"SlrR Streak plate Natto", "category":"sl", "number": 25, "isroot": false, "next": null }, { "date":"02-09-2015", "title":"SlrR Streak plate Natto", "category":"sl", "number": 26, "isroot": false, "next": ["sl27"] }, { "date":"09-09-2015", "title":"SlrR Natto biofilms", "category":"sl", "number": 27, "isroot": false, "next": []

       },
       {

"date":"16-04-2015", "title":"tasA Primer design", "category":"t", "number": 1, "isroot": true, "next": ["t2","t4"] }, { "date":"13-05-2015", "title":"tasA PCR", "category":"t", "number": 2, "isroot": false, "next": ["t3"] }, { "date":"13-05-2015", "title":"tasA PCR purification", "category":"t", "number": 3, "isroot": false, "next": null }, { "date":"15-05-2015", "title":"tasA PCR", "category":"t", "number": 4, "isroot": false, "next": ["t5"] }, { "date":"18-05-2015", "title":"tasA PCR purification", "category":"t", "number": 5, "isroot": false, "next": ["t6","t7"] }, { "date":"18-05-2015", "title":"tasA Assembly PCR", "category":"t", "number": 6, "isroot": false, "next": null }, { "date":"19-05-2015", "title":"tasA Gibson Assembly", "category":"t", "number": 7, "isroot": false, "next": ["t8"] }, { "date":"19-05-2015", "title":"tasA PCR purification", "category":"t", "number": 8, "isroot": false, "next": ["t9"] }, { "date":"19-05-2015", "title":"tasA PCR", "category":"t", "number": 9, "isroot": false, "next": ["t10"] }, { "date":"20-05-2015", "title":"tasA PCR purification", "category":"t", "number": 10, "isroot": false, "next": ["t11"] }, { "date":"20-05-2015", "title":"tasA Digestion", "category":"t", "number": 11, "isroot": false, "next": ["t13"] }, { "date":"30-04-2015", "title":"Backbone Digestion", "category":"t", "number": 24, "isroot": true, "next": ["t25"] }, { "date":"30-04-2015", "title":"Backbone miniprep", "category":"t", "number": 25, "isroot": false, "next": ["t13","t26"] }, { "date":"27-05-2015", "title":"Backbone Digestion", "category":"t", "number": 26, "isroot": false, "next": ["t27"] }, { "date":"27-05-2015", "title":"Backbone Digestion", "category":"t", "number": 27, "isroot": false, "next": null }, { "date":"20-05-2015", "title":"tasA PCR purification", "category":"t", "number": 13, "isroot": false, "next": ["t14","t21","t28"] }, { "date":"21-05-2015", "title":"tasA Gel purification", "category":"t", "number": 14, "isroot": false, "next": ["t15"] }, { "date":"21-05-2015", "title":"tasA Ligation", "category":"t", "number": 15, "isroot": false, "next": ["t16"] }, { "date":"21-05-2015", "title":"tasA Heat shock transformation", "category":"t", "number": 16, "isroot": false, "next": ["t18"] }, { "date":"27-05-2015", "title":"tasA Miniprep", "category":"t", "number": 18, "isroot": false, "next": ["t19"] }, { "date":"28-05-2015", "title":"tasA Digestion", "category":"t", "number": 19, "isroot": false, "next": null }, { "date":"02-06-2015", "title":"tasA Digestion", "category":"t", "number": 20, "isroot": false, "next": ["t21"] }, { "date":"02-06-2015", "title":"tasA Gel purification", "category":"t", "number": 21, "isroot": false, "next": ["t22"] }, { "date":"03-06-2015", "title":"tasA Ligation", "category":"t", "number": 22, "isroot": false, "next": ["t23"] }, { "date":"03-06-2015", "title":"tasA Transformation", "category":"t", "number": 23, "isroot": false, "next": null }, { "date":"08-06-2015", "title":"tasA PCR", "category":"t", "number": 28, "isroot": false, "next": ["t29"] }, { "date":"08-06-2015", "title":"tasA PCR purification", "category":"t", "number": 29, "isroot": false, "next": ["t30"] }, { "date":"09-06-2015", "title":"tasA Digestion", "category":"t", "number": 30, "isroot": false, "next": ["t31"] }, { "date":"09-06-2015", "title":"tasA PCR purification", "category":"t", "number": 31, "isroot": false, "next": ["t32"] }, { "date":"09-06-2015", "title":"tasA Ligation", "category":"t", "number": 32, "isroot": false, "next": ["t33"] }, { "date":"09-06-2015", "title":"tasA Heat shock transformation", "category":"t", "number": 33, "isroot": false, "next": ["t34"] }, { "date":"11-06-2015", "title":"tasA Miniprep", "category":"t", "number": 34, "isroot": false, "next": ["t35","t36","t37"] }, { "date":"11-06-2015", "title":"tasA Digestion", "category":"t", "number": 35, "isroot": false, "next": null }, { "date":"15-06-2015", "title":"tasA Digestion", "category":"t", "number": 36, "isroot": false, "next": null }, { "date":"16-06-2015", "title":"tasA Digestion", "category":"t", "number": 37, "isroot": false, "next": ["t38"] }, { "date":"18-06-2015", "title":"tasA Sequencing", "category":"t", "number": 38, "isroot": false, "next": ["t39"] }, { "date":"08-07-2015", "title":"tasA Digestion pSB1C3", "category":"t", "number": 39, "isroot": false, "next": ["t40"] }, { "date":"08-07-2015", "title":"tasA PCR purification", "category":"t", "number": 40, "isroot": false, "next": ["t41"] }, { "date":"08-07-2015", "title":"tasA Ligation pSB1C3", "category":"t", "number": 41, "isroot": false, "next": ["t42"] }, { "date":"08-07-2015", "title":"tasA Transformation", "category":"t", "number": 42, "isroot": false, "next": ["t43"] }, { "date":"10-07-2015", "title":"tasA Miniprep", "category":"t", "number": 43, "isroot": false, "next": ["t44","t69","t61","t75"] }, { "date":"16-07-2015", "title":"tasA Digestion K23", "category":"t", "number": 44, "isroot": false, "next": ["t45","t52","t56"] }, { "date":"16-07-2015", "title":"tasA PCR purification", "category":"t", "number": 45, "isroot": false, "next": ["t46"] }, { "date":"16-07-2015", "title":"tasA Ligation", "category":"t", "number": 46, "isroot": false, "next": ["t47"] }, { "date":"16-07-2015", "title":"tasA Transformation", "category":"t", "number": 47, "isroot": false, "next": ["t48"] }, { "date":"17-07-2015", "title":"tasA Miniprep", "category":"t", "number": 48, "isroot": false, "next": ["t49","t50"] }, { "date":"21-07-2015", "title":"tasA Transformation", "category":"t", "number": 49, "isroot": false, "next": null }, { "date":"20-07-2015", "title":"tasA Sequencing", "category":"t", "number": 50, "isroot": false, "next": ["t69","t61"] }, { "date":"23-07-2015", "title":"tasA Digestion Psalt", "category":"t", "number": 51, "isroot": true, "next": ["t52"] }, { "date":"23-07-2015", "title":"tasA PCR purification", "category":"t", "number": 52, "isroot": false, "next": ["t53"] }, { "date":"23-07-2015", "title":"tasA Ligation", "category":"t", "number": 53, "isroot": false, "next": ["t54","t55"] }, { "date":"31-07-2015", "title":"tasA Colony PCR", "category":"t", "number": 54, "isroot": false, "next": null }, { "date":"23-07-2015", "title":"tasA Transformation", "category":"t", "number": 55, "isroot": false, "next": null }, { "date":"28-07-2015", "title":"tasA Digestion", "category":"t", "number": 56, "isroot": false, "next": ["t57"] }, { "date":"28-07-2015", "title":"tasA PCR purification", "category":"t", "number": 57, "isroot": false, "next": ["t58","t59"] }, { "date":"28-07-2015", "title":"tasA Ligation", "category":"t", "number": 58, "isroot": false, "next": ["t60"] }, { "date":"30-07-2015", "title":"tasA Sequencing", "category":"t", "number": 59, "isroot": false, "next": [] }, { "date":"29-07-2015", "title":"tasA Transformation", "category":"t", "number": 60, "isroot": false, "next": null }, { "date":"06-08-2015", "title":"tasA Digestion", "category":"t", "number": 61, "isroot": false, "next": ["t62"] }, { "date":"06-08-2015", "title":"tasA Digestion", "category":"t", "number": 62, "isroot": false, "next": ["t63"] }, { "date":"06-08-2015", "title":"tasA PCR purification", "category":"t", "number": 63, "isroot": false, "next": ["t64"] }, { "date":"06-08-2015", "title":"tasA Ligation", "category":"t", "number": 64, "isroot": false, "next": ["t65"] }, { "date":"07-08-2015", "title":"tasA Transformation", "category":"t", "number": 65, "isroot": false, "next": ["t66"] }, { "date":"09-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 66, "isroot": false, "next": ["t67"] }, { "date":"11-08-2015", "title":"tasA Miniprep", "category":"t", "number": 67, "isroot": false, "next": null }, { "date":"13-08-2015", "title":"tasA Digestion", "category":"t", "number": 69, "isroot": false, "next": ["t70"] }, { "date":"13-08-2015", "title":"tasA PCR purification", "category":"t", "number": 70, "isroot": false, "next": ["t71"] }, { "date":"13-08-2015", "title":"tasA Ligation", "category":"t", "number": 71, "isroot": false, "next": ["t72"] }, { "date":"14-08-2015", "title":"tasA Transformation", "category":"t", "number": 72, "isroot": false, "next": ["t73","t74"] }, { "date":"17-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 73, "isroot": false, "next": null }, { "date":"18-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 74, "isroot": false, "next": null }, { "date":"17-08-2015", "title":"tasA Digestion", "category":"t", "number": 75, "isroot": false, "next": ["t76"] }, { "date":"17-08-2015", "title":"tasA Purification", "category":"t", "number": 76, "isroot": false, "next": ["t77"] }, { "date":"17-08-2015", "title":"tasA Ligation Psalt", "category":"t", "number": 77, "isroot": false, "next": ["t78"] }, { "date":"17-08-2015", "title":"tasA Transformation", "category":"t", "number": 78, "isroot": false, "next": ["t102"] }, { "date":"18-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 102, "isroot": false, "next": ["t100"] }, { "date":"19-08-2015", "title":"tasA Miniprep", "category":"t", "number": 100, "isroot": false, "next": ["t93","t84","t79"] }, { "date":"19-08-2015", "title":"tasA Digestion", "category":"t", "number": 79, "isroot": false, "next": ["t80"] }, { "date":"19-08-2015", "title":"tasA Purification", "category":"t", "number": 80, "isroot": false, "next": ["t81"] }, { "date":"20-08-2015", "title":"tasA Ligation Psalt", "category":"t", "number": 81, "isroot": false, "next": ["t82"] }, { "date":"20-08-2015", "title":"tasA Transformation", "category":"t", "number": 82, "isroot": false, "next": ["t83"] }, { "date":"21-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 83, "isroot": false, "next": null }, { "date":"24-08-2015", "title":"tasA Digestion", "category":"t", "number": 84, "isroot": false, "next": ["t85"] }, { "date":"24-08-2015", "title":"tasA Purification", "category":"t", "number": 85, "isroot": false, "next": ["t86"] }, { "date":"24-08-2015", "title":"tasA Ligation Psalt", "category":"t", "number": 86, "isroot": false, "next": ["t87"] }, { "date":"24-08-2015", "title":"tasA Transformation", "category":"t", "number": 87, "isroot": false, "next": ["t88"] }, { "date":"26-08-2015", "title":"tasA Miniprep", "category":"t", "number": 88, "isroot": false, "next": ["t89"] }, { "date":"26-08-2015", "title":"tasA Digestion", "category":"t", "number": 89, "isroot": false, "next": ["t90"] }, { "date":"26-08-2015", "title":"tasA Purification", "category":"t", "number": 90, "isroot": false, "next": ["t91"] }, { "date":"26-08-2015", "title":"tasA Ligation", "category":"t", "number": 91, "isroot": false, "next": ["t92"] }, { "date":"27-08-2015", "title":"tasA Transformation", "category":"t", "number": 92, "isroot": false, "next": null }, { "date":"28-08-2015", "title":"tasA Digestion", "category":"t", "number": 93, "isroot": false, "next": ["t94"] }, { "date":"28-08-2015", "title":"tasA Purification", "category":"t", "number": 94, "isroot": false, "next": ["t95"] }, { "date":"28-08-2015", "title":"tasA Ligation", "category":"t", "number": 95, "isroot": false, "next": ["t96"] }, { "date":"28-08-2015", "title":"tasA Transformation", "category":"t", "number": 96, "isroot": false, "next": ["t97"] }, { "date":"29-08-2015", "title":"tasA Colony PCR", "category":"t", "number": 97, "isroot": false, "next": ["t98"] }, { "date":"30-08-2015", "title":"tasA Miniprep", "category":"t", "number": 98, "isroot": false, "next": ["t99"] }, { "date":"31-08-2015", "title":"tasA Transformation", "category":"t", "number": 99, "isroot": false, }, { "date":"25-08-2015", "title":"Grow E. coli pdg", "category":"th", "number": 1, "isroot": true, "next": ["th2"] }, { "date":"26-08-2015", "title":"Miniprep", "category":"th", "number": 2, "isroot": false, "next": ["th3"] }, { "date":"26-08-2015", "title":"Restriction", "category":"th", "number": 3, "isroot": false, "next": ["th4"] }, { "date":"26-08-2015", "title":"Ligation", "category":"th", "number": 4, "isroot": false, "next": ["th5"] }, { "date":"26-08-2015", "title":"Transform k23", "category":"th", "number": 5, "isroot": false, "next": ["th6"] }, { "date":"27-08-2015", "title":"Liquid LB", "category":"th", "number": 6, "isroot": false, "next": ["th7"] }, { "date":"27-08-2015", "title":"Miniprep", "category":"th", "number": 7, "isroot": false, "next": ["th8"] }, { "date":"27-08-2015", "title":"Restriction Analysis", "category":"th", "number": 8, "isroot": false, "next": null }, { "date":"25-08-2015", "title":"Grow k23", "category":"th", "number": 9, "isroot": true, "next": ["th10"] }, { "date":"26-08-2015", "title":"Miniprep", "category":"th", "number": 10, "isroot": false, "next": ["th11"] }, { "date":"26-08-2015", "title":"Restriction", "category":"th", "number": 11, "isroot": false, "next": ["th4"] }, { "date":"25-08-2015", "title":"Ordered Primers", "category":"th", "number": 12, "isroot": true, "next": ["th13"] }, { "date":"02-09-2015", "title":"PCR thrC 2", "category":"th", "number": 13, "isroot": false, "next": ["th14"] }, { "date":"03-09-2015", "title":"Restriction", "category":"th", "number": 14, "isroot": false, "next": ["th15"] }, { "date":"03-09-2015", "title":"PCR Purification", "category":"th", "number": 15, "isroot": false, "next": ["th16"] }, { "date":"03-09-2015", "title":"Ligation", "category":"th", "number": 16, "isroot": false, "next": ["th17"] }, { "date":"03-09-2015", "title":"Transformation", "category":"th", "number": 17, "isroot": false, "next": ["th18"] }, { "date":"04-09-2015", "title":"Colony PCR", "category":"th", "number": 18, "isroot": false, "next": ["th19"] }, { "date":"05-09-2015", "title":"Liquid Medium", "category":"th", "number": 19, "isroot": false, "next": ["th20"] }, { "date":"05-09-2015", "title":"Miniprep", "category":"th", "number": 20, "isroot": false, "next": ["th21"] }, { "date":"06-09-2015", "title":"Restriction with Inserts", "category":"th", "number": 21, "isroot": false, "next": ["th22"] }, { 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]