Team:Concordia/ResultsCloning

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  • Lactococcus Lactis
  • Scaffold
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Results of Cloning

Gel Electrophoresis of Team Parts

We successfully cloned the 8 components (registry parts BBa_K1830001 to BBa_K1830008) making up the scaffold, as seen in gel lanes 1 to 16, into a single construct and the 2 dockerin parts Dock1 and XDock2 (BBa_K1830009 and BBa_K1830010), as seen in lanes 17 to 20, into their respective pSB1C3 backbones. Gel lanes 21 to 26 showcase 3 enzymes, SacC, ADH2, ALD2, that were cloned into backbones. In total, 13 original parts were added by our team to the iGEM Parts registry.

Gel Lanes	Part	Registry #
1,2	PnisA	BBa_K1830001
3,4	SPusp45	BBa_K1830002
5,6	CBD	BBa_K1830003
7,8	Coh1c3	BBa_K1830004
9,10	Coh2S1	BBa_K1830005
11,12	CWAm6	BBa_K1830006
13,14	T1T2 terminator	BBa_K1830007
15,16	Lactis shuttle	BBa_K1830008
17,18	Dock1	BBa_K1830009
19,20	XDock2	BBa_K1830010
21,22	SacC	BBa_K1830015
23,24	ADH2 (alcohol dehydrogenase)	BBa_K1830013
25,26	ALD2 (aldehyde dehydrogenase)	BBa_K1830014