Team:SF Bay Area DIYBio/Results

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Project Results

UV kill curve was generated for E.coli HB101, to establish baseline exposure for Directed Evolution We also tested HB101 + pGLO plasmid, to check if GFP has a protective effect.

4 log decrease in viable cells after 75min for HB101, 30min for pGLO If anything, pGLO has a negative effect on UV resistance!

Accomplishments

Researched UV sources and built UV exposure rig to mimic solar UV

Determined exposure level needed for >4 log decrease in CFU

Demonstrated GFP is NOT an effective UV protectant for E.coli

Synthesized and transformed A. variabilis shinorine biosynthesis genes

Submitted one gene (Ava_3856) to Parts Registry

Demonstrated that we produced UV absorbing compounds