Team:Concordia/Notebook
Notebook
Week 1
Wet lab work:
Tuesday: -Initial PCR for coh2S1 and CWAm6 parts from pAW576 -Purification and digestion of the parts and the backbone with EcoRI and PstI
Wednesday: -Initial PCR for PnisA and SPusp45 from pAW576 and IDT G-Blocks, only PnisA was amplified -Initial PCR for CBD and coh2S1 from ordered IDT G-Blocks (group 2), not successful
Thursday: -Ligation and transformation attempt of the plasmids containing coh2S1 and CWAm6 following the iGEM protocol, but with small concentrations and no results -Second attempt at amplifying the G-Block parts with different primers, no success -Digest of amplified and purified PnisA with EcoRI and PstI and of the backbone
Friday: -Blunt-end cloning to amplify the G-Block parts from a pJET vector -PCR amplification, digest, ligation and transformation of coh2S1 and CWAm6, no results
Saturday: -Second attempt at the same ligation using a new digested backbone and a new T4 ligase
Sunday: -No results from Saturday and probably some contamination on the plates.
Dry lab work:
Monday: -Research on our research subject and our project -Preparation of a presentation to our supervisors and professors about our project organization and timeline
Tuesday: -Presentation in front of our supervisors and professors
Wednesday: -Sponsorship package
Thursday: -Sponsorship package
Friday: -Restriction enzymes order received
Saturday: -Sponsorship package -Talk about fundraising ideas
Sunday: -Sponsorship package -First thoughts on a bake sale soon
Tuesday: -Initial PCR for coh2S1 and CWAm6 parts from pAW576 -Purification and digestion of the parts and the backbone with EcoRI and PstI
Wednesday: -Initial PCR for PnisA and SPusp45 from pAW576 and IDT G-Blocks, only PnisA was amplified -Initial PCR for CBD and coh2S1 from ordered IDT G-Blocks (group 2), not successful
Thursday: -Ligation and transformation attempt of the plasmids containing coh2S1 and CWAm6 following the iGEM protocol, but with small concentrations and no results -Second attempt at amplifying the G-Block parts with different primers, no success -Digest of amplified and purified PnisA with EcoRI and PstI and of the backbone
Friday: -Blunt-end cloning to amplify the G-Block parts from a pJET vector -PCR amplification, digest, ligation and transformation of coh2S1 and CWAm6, no results
Saturday: -Second attempt at the same ligation using a new digested backbone and a new T4 ligase
Sunday: -No results from Saturday and probably some contamination on the plates.
Dry lab work:
Monday: -Research on our research subject and our project -Preparation of a presentation to our supervisors and professors about our project organization and timeline
Tuesday: -Presentation in front of our supervisors and professors
Wednesday: -Sponsorship package
Thursday: -Sponsorship package
Friday: -Restriction enzymes order received
Saturday: -Sponsorship package -Talk about fundraising ideas
Sunday: -Sponsorship package -First thoughts on a bake sale soon