Template:Team:Groningen/CONTENT/LOGBOOK/Restriction with Inserts th21
Restriction thrC compleet, tasA, bslA and slrR Inserts (th21)
Digestion of thrC completed
Extra integration of DNA in thrC locus
Digested products
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 6 September 2015 - 00:00, 6 September 2015
The following components were used.
Construct
Restriction enzymes
ThrC backbone
EcoRI and PstI
Restriction.
The restriction was performed for 2 hours at 37 °C in stopped by heating at 80°C for 20 min.