The starter culture from 7/11 overgrew to around an OD of 3 (want OD around 0.5) so I am re-picking colonies to make a new starter culture in 7 ml of LB broth. Put in 37 C incubator t 9:40 AM
Using [http://www.zymoresearch.com/downloads/dl/file/id/166/t3001i.pdf this] protocol.
At 1 pm (3 h 20 m) the OD was up to 0.52, (shooting for between 0.4 and 0.6)
Added 0.5 ml to 50 ml of zymo broth. Left at RT for an hour and placed into 21 C incubator starting at 2 pm 7/12.
The incubator started at around 27 C at 2 pm and is taking a while to ramp down.
At 5:50 Pm the OD is 0.087, which seems rather high for only 4 hours. The protocol says 10 to 36 hours.