Team:IIT Kharagpur/Notebook

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("Log of day-to-day work done")

WEEK-1( July 6 - July 10 )

6th July 2015

LB media prepared (500 ml), autoclaved and stored at 4 degrees.
250 ml of LB agar prepared and poured into the plates (chloramphenicol antibiotic used- concentration 1 µl/ml).

7th July 2015

DNA from wells dissolved into 10 µl distilled water. DNA kit plate instructions
Transformed 4µl of DNA into 100µl of E.Coli DH5α. transformation procedure
100µl of transformed cells were plated on chloramphenicol resistant plates.and left overnight to grow.

8th July 2015

Colonies for luxPR promoter (BBa_K546003) constructs were observed. A colony was picked and inoculated in 3 ml culture for glycerol stock preparation.
No colony observed for luxR protein (BBa_R6002) construct.

9th July 2015

Transformation repeated for luxR protein construct. transformation procedure
Culture prepared for plasmid isolation for luxPR promoter. Colony was picked and inoculated in 10 ml culture.

10th July 2015

No colony observed for luxR protein construct.
Plasmid isolation done for luxPR promoter.
Gel electrophoresis done for luxPR promoter.
Lane 7 : 1kb marker
Lane 8 : luxPR promoter