Team:Paris Saclay/Notebook/July/9

Wednesday 8th July

Lab Work

Transformation

by Coralie

Ligation product:

  • J23101 + I13504
  • J23106 + I13504
  • J23117 + I13504

On LB + Ampicillin 100ug/mL Incubation ON, 37°C

"by Johan and Seong Koo"

R0051 On LB + Ampicillin 100ug/mL Incubation ON, 37°C

Plasmid Rehydratation :

by Johan and Audrey

Biobricks:

  • S03518
  • B0030
  • B0015
  • K1399005

Digestion

by Pauline and Audrey

Plasmid with promotor: J23101 10uL of our plasmid with promotor 1uL SpeI 1uL PstI 2uL buffer 10x FD

Plasmid with gene: COO40 and K115017 10uL of our plasmid with gene 1uL XbaI 1uL PstI 2uL buffer 10x FD

After this step, we separate the sequence we need from the sequence we don't with electrophoresis

Electrophoresis

by Pauline and Audrey

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

Quantification

by Pauline and Audrey We quantify with the electrophoresis gel: C0040: 10ng/uL J23101: 20ng/uL K115017: we don't see enough of fluorescent to quantify the biobrick. We decide to amplify this one by PCR.

Members present:

  • Instructors and advisors: Alice.
  • Students: Johan, Seong Koo, Audrey, Coralie, Pauline