Team:NTNU Trondheim
Welcome to the NTNU Trondheim iGEM 2015 webpage!
NTNU Trondheim iGEM 2015
NTNU Trondheim is a team from Trondheim, Norway, competing for the iGEM 2015 student competition. NTNU Trondheim maintains the iGEM Matchmaker Tool which helps iGEM teams cooperate with each other.
We are planning to engineer Pseudomonas putida to function as a glucose detector. In the presence of glucose it will express mCherry. To achieve this we will use promoters of four operons commonly found in Pseudomonas. The operons are negatively controlled and expression of subsequent genes is initiated by derivatives of glucose. We will characterize the performance of these four promoters and choose one to do further work on. Further, we will encapsulate our engineered P. putida in alginate. It is a biopolymer from brown algae that can be used to form gel capsules. Alginate is permeable for small molecules like glucose and shields the encapsulated cells from the surroundings.