Team:Paris Saclay/Notebook/July/7

Tuesday 7th July

Back in Lab

Verification of our plasmids

by Coralie, Johan, Seong Koo, Audrey, Pauline

Biobricks verification:
  • K115017
  • C0040
  • I13602
  • J23101
  • J23117
  • J23106
  • I13504
  • K1493504
Extraction

With the Nucleospin Kit from Macherey Nagel

Digestion by NotI:

Stock solution preparation :

  • 124µL H2O
  • 16µL buffer FastDigest 10x
  • 4µL NotI

We use 18µL from this solution with 2µL plasmid solution

Incubation 1h30 at 37°C

Electrophoresis

by Johan, Audrey, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V


New culture

by Pauline, Audrey

New liquid culture of:

  • K1493504

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.


Members present:

  • Instructors and advisors: Alice.
  • Students: Johan, Seong Koo, Audrey, Coralie, Pauline