Team:Reading/Notebook

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Lab Book

Dates are in the English format DD/MM/YY

29/06/15

Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of .6803 WT.

Cultured 5 streak plates of .6803 on solid BG-11

All were placed on the windowsill in our lab in natural light

02/07/15

Three new 250ml conical flasks were set up on the shaker (42rpm). Each contained 5 loopfuls of .6803 WT and 100ml of BG-11. They were exposed to 24hr light by the overhead lights in the lab.

07/07/15

Flask 5 cultured containing 10ul .6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements.

10/07/15

2 Litres of water collected from Reading University Lake for SODIS, method outlines in protocols.

13/07/15

Estimating the Cells per ml for the flask from 29/06 1a.u at 750nm = 1.6x10⁸

OD₇₅₀ = 0.028

So

0.028x1.6x10⁸ = 4.48x10⁶ cells per ml

Document the dates you worked on your project.

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