Lab Book
Dates are in the UK format DD/MM/YY
29/06/15
Cultured a 250ml conical flask using 100 ml BG-11 and 5 loopfuls of .6803 WT.
Cultured 5 streak plates of .6803 on solid BG-11
All were placed on the windowsill in our lab in natural light
02/07/15
Three new 250ml conical flasks were set up on the shaker (42rpm). Each contained 5 loopfuls of .6803 WT and 100ml of BG-11. They were exposed to 24hr light by the overhead lights in the lab.
07/07/15
Flask 5 cultured containing 10ul .6803 WT in 100ml BG-11. This flask will be used for preliminary growth curve measurements. The flask was placed on the shaker with flasks 2,3 and 4.
| Date | OD750 | Estimated Cells per ml |
| 07/07 | 0.000 | 0 |
| 08/07 | 0.003 | 4.8x105 |
| 09/07 | 0.005 | 8x105 |
| 10/07 | 0.005 | 8x105 |
| 13/07 | 0.005 | 8x105 |
| 14/07 | 0.112 | 1.792x107 |
| 15/07 | 0.008 | 1.28x106 |
| 16/07 | 0.009 | 1.44x106 |
| 17/07 | 0.03 | 4.8x106 |
10/07/15
2 Litres of water collected from Whiteknights Lake on campus for SODIS, method outlines in protocols.
13/07/15
Estimating the Cells per ml for the flask from 29/06 1a.u at 750nm = 1.6x108
OD750 = 0.028 So 0.028x1.6x108 = 4.48x106 cells per ml
14/07/15
The lamp safety testing was completed so our flasks were placed under the white light bulb
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- Chronological notes of what your team is doing.
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