Team:EPF Lausanne/Notebook/Protocols
Protocols
Agarose Gel Preparation
1,2% agarose gel – for small DNA fragments
Mix 50 mL 1X TAE and 0.6 g Agarose
Melt in microwave until agarose has melted (about 50 seconds)
Add 1.3 μL Gel Red
Pour solution into agarose gel mold with comb
Let set for 20 minutes or until solid
Place gel in 1X TAE and remove comb
Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and water up to 12 μL
Run gel at 100-120 Volts for 40-50 minutes (depending on size of fragments)
Take a picture of the gel at the UV detector
3% agarose gel – for large DNA fragments
Mix 50 mL 1X TAE and 1.5 g Agarose
Melt in microwave until agarose has melted (about 50 seconds)
Add 1.5 μL Gel Red
Pour solution into agarose gel mold with comb
Let set for 20 minutes or until solid
Place gel in 1X TAE and remove comb
Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and water up to 12 μL
Run gel at 80 Volts for 2 hours
Take a picture of the gel at the UV detector
Section 2
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Section 3
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Section 4-1
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Section 4-2
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