Team:EPF Lausanne/Notebook/Protocols
Protocols
Agarose Gel Preparation
1,2% agarose gel – for small DNA fragments
• Mix 50 mL 1X TAE and 0.6 g Agarose
• Melt in microwave until agarose has melted (about 50 seconds)
• Add 1.3 μL Gel Red
• Pour solution into agarose gel mold with comb
• Let set for 20 minutes or until solid
• Place gel in 1X TAE and remove comb
• Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and water up to 12 μL
• Run gel at 100-120 Volts for 40-50 minutes (depending on size of fragments)
• Take a picture of the gel at the UV detector
3% agarose gel – for large DNA fragments
• Mix 50 mL 1X TAE and 1.5 g Agarose
• Melt in microwave until agarose has melted (about 50 seconds)
• Add 1.5 μL Gel Red
• Pour solution into agarose gel mold with comb
• Let set for 20 minutes or until solid
• Place gel in 1X TAE and remove comb
• Load samples of 200 ng (or 2 μL) DNA mixed with 2 μL 6X loading dye and water up to 12 μL
• Run gel at 80 Volts for 2 hours
• Take a picture of the gel at the UV detector
Section 2
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Section 3
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Section 4-1
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Section 4-2
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