Team:Paris Saclay/Notebook/July/9
Contents
Thursday 9th July
Lab Work
Transformation
by Coralie
Ligation product:
- BBa_J23101 + BBa_I13504
- BBa_J23106 + BBa_I13504
- BBa_J23117 + BBa_I13504
On LB + Ampicillin 100ug/mL. Incubation ON, 37°C
by Johan and Seong Ko
- BBa_R0051
On LB + Ampicillin 100ug/mL. Incubation ON, 37°C
Plasmid Rehydratation
by Johan and Audrey
- BBa_S03518
- BBa_B0030
- BBa_B0015
- BBa_K1399005
Digestion
by Pauline and Audrey
Plasmid with promotor: BBa_J23101
- 10µL of our plasmid with promotor
- 1µL SpeI
- 1µL PstI
- 2µL buffer 10x FD
Plasmid with gene: BBa_COO40 and BBa_K115017
- 10µL of our plasmid with gene
- 1µL XbaI
- 1µL PstI
- 2µL buffer 10x FD
After this step, we separate the sequence we need from the sequence we don't with electrophoresis
Electrophoresis
by Pauline and Audrey
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V
Quantification
by Pauline and Audrey
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V We quantify with the electrophoresis gel:
- BBa_C0040: 10ng/µL
- BBa_J23101: 20ng/µL
- BBa_K115017: we don't see enough of fluorescent to quantify the biobrick. We decide to amplify this one by PCR.
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Audrey, Coralie, Pauline
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.