Team:Paris Saclay/Notebook/July/17

Friday 17th July

Lab Work

Ligation

by Pauline

In each tube:

  • 3 µL Buffer DNA pol T4
  • 1µL DNA pol T4
  • 4µL H2O
  • BBa_J23101 (10µL) + BBa_S03518 (12µL)
  • BBa_S03518 (12µL) + BBa_B0015 (10µL)
  • BBa_B0015 (10µL) + BBa_I13602 (12µL)

Incubation 4h, 4°C

New culture

by Coralie

  • 1696: Bad::Tet
  • 1320:ClpP::Cm
  • 1693:z1:Spect

On LB plates, incubation ON, 37°C

Transformation

by Johan

Transformation with ligation product:

  • BBa_J23101 + BBa_S03518 = BBa_K1707001
  • BBa_B0015 + BBa_I13602 = BBa_K1707002
  • BBa_S03518 + BBa_B0015 = BBa_K1707003

New culture on plates

by Pauline

  • BBa_J23101 + BBa_I13504
  • BBa_J23106 + BBa_I13504
  • BBa_J23117 + BBa_I13504
  • BBa_B0030
  • BBa_R0051

Plasmid extraction

by Pauline and Seong-Koo

  • BBa_K1707000
  • BBa_K1399019
  • BBa_K1399005
  • BBa_K1399023

Cultures from the 16/15/2015 With the Nucleospin Kit from Macherey Nagel

Members present:

  • Instructors and advisors: Alice.
  • Students: Johan, Seong Koo, Coralie, Pauline