Team:UCLA/Notebook/Protein Cages/21 July 2015

Nithin's Notes

PCR to add the iGEM suffix to our PcQuad was done. We used igem prefix 1 and igem suffix 2 to run the reaction. The template DNA was obtained through the dna extraction that was done yesterday. This product was diluted to give us a 20ul tube of 1ng/ul. I loaded 500 ng of template for a 50 ul reacton. After running the gel, we werent able to see the band that we wanted. But, we did see the same nonspecific band that had haunted us earlier shining ever so brightly.

I did change the annealing time from 25 seconds to 30 seconds for this reaction.

Lane 1: Ladder

Lane 2-5: 25ul of the same reaction with same conditions.