Team:RHIT/Notebook
Notebook
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
6/8/15-6/12/15
6/11/15
p416-GPD was streaked on LB+Amp plate
6/15/15-6/19/15
6/14/15
Four overnight cultures of p416-GPD colonies were started.
6/15/15
We performed minipreps on overnight cultures of p416 and recovered eight aliquots of 50 µL. Miniprep #s 1, 3, 5, and 7 were digested with EcoR1. When run on a .9% gel, confirmed that minipreps are concentrated enough to use.
6/16/15
IDT constructs 1 and 2 were resuspended to 10 ng/µL.
6/17/15
The iGEM shipping vector, pSB1C3, and construct 2 (the translational unit) were digested with EcoRI and PstI and the resulting fragments were ligated. The NEBuilder procedure was also used to assemble ligated pSB1C3 and construct 2 from IDT. Both NEBuilder assembled, and ligation assembled plasmids were transformed with E. coli and 100 µL aliquots were plated on LB+chlor plates.
6/18/15
Construct 2 and p416 (from miniprep #1) were digested with SpeI and XbaI. Digests were purified using the QIAquick Gel Extraction Kit.
6/19/15
The gel purified fragments from yesterday were concentrated by ethanol precipitation, succeeded by resuspension in TE. V was resuspended in 10 µL and I was resuspended in 40 µL. The entire 10 µL V was ligated with 7 µL I and transformed into E. coli.
More plates of the pSB1C3/T.U. ligation and NEBuilder Assembly were plated (2 plates with 300 µL each).
6/22/15-6/26/15
6/22/15
p416-GPD was streaked on LB+Amp plate
6/23/15
p416-GPD was streaked on LB+Amp plate
6/24/15
p416-GPD was streaked on LB+Amp plate
6/25/15
p416-GPD was streaked on LB+Amp plate
6/26/15
p416-GPD was streaked on LB+Amp plate
6/29/15-7/3/15
6/28/15
p416-GPD was streaked on LB+Amp plate
6/29/15
p416-GPD was streaked on LB+Amp plate
6/30/15
p416-GPD was streaked on LB+Amp plate
7/1/15
p416-GPD was streaked on LB+Amp plate
7/2/15
p416-GPD was streaked on LB+Amp plate
7/3/15
p416-GPD was streaked on LB+Amp plate
7/6/15-7/10/15
This week was taken off as a team break
7/13/15-7/17/15
7/13/15
p416-GPD was streaked on LB+Amp plate
7/14/15
p416-GPD was streaked on LB+Amp plate
7/15/15
p416-GPD was streaked on LB+Amp plate
7/16/15
p416-GPD was streaked on LB+Amp plate
7/17/15
p416-GPD was streaked on LB+Amp plate
7/20/15-7/24/15
7/20/15
p416-GPD was streaked on LB+Amp plate
7/21/15
p416-GPD was streaked on LB+Amp plate
7/22/15
p416-GPD was streaked on LB+Amp plate
7/23/15
p416-GPD was streaked on LB+Amp plate
7/24/15
p416-GPD was streaked on LB+Amp plate
8/3/15-8/7/15
8/10/15-8/14/15
8/17/15-8/21/15